Instructions for Use:

  1. Remove the required number of ampoules from the frame. Be careful not to damage the foil of remaining ampoules.
  2. Open ampoules by punching a hole in the aluminum foil with the corner of the ampoule frame or a pair of scissors. Mark the ampoules with a number for sample identification.
  3. Take a fresh disposable pipette for each milk sample. Do not re-use pipettes. Do not touch the tip-end, which will be in contact with the milk.
  4. Add the milk into the pipette by squeezing the smaller upper bulb once, hold it, dip the pipette tip about 1 cm into the milk sample. Then, release pressure on the bulb and the pipette (stem) will fill itself with the appropriate volume (0.1 ml) of milk.
  5. Transfer the milk sample by gently and completely squeezing the same upper bulb, adding the milk straight onto the agar medium. Note: After pipetting, a small amount of milk will remain in the pipette reservoir (the small lower bulb).
  6. Check that the temperature of the incubator is (64°C +/- 2°C). Place the ampoules into the incubator. Record the time and set the timer for 3 hours (SP-NT), 3 hours 15 minutes (T)
  7. Read the colour of the solid agar in the ampoules after the required incubation time.

Points of Attention:

  1. Refer to the colour card for the correct yellow colour indicating negative. The incubation time for sheep milk takes approximately 10 minutes more than goat or cow milk.
  2. To allow more time for reading after incubation, the tests can be dipped in a cold bath of water with ice. The cold stops further colour change.
  3. The 1/3 upper part of the agar gel in the ampoule might remain purple while the 2/3 lower part of the gel is yellow. The test result is then negative.
  4. Do not use the pipette from the Delvotest BLF test.

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